IKK alpha Antibody

Product Specifications

Background

IKK alpha Antibody: Nuclear factor kappa B (NF-κ B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κ B is associated with Iκ B proteins in the cell cytoplasm, which inhibit NF-κ B activity. The long-sought Iκ B kinase (IKK), which phosphorylates Iκ B, and mediates Iκ B degradation and NF-κ B activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ ) . IKKα and IKKβ interact with each other and both are essential for the NF-κ B activation. IKKα specifically phosphorylates IkB-alpha. IKKα is expressed in variety of human tissues.

NCBI Gene ID

1147

Swiss Prot

O15111

Accession Number

AF009225

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Overexpression validation (Figure 8) : ASC expression detected by anit-ASC antibodies (2287) was shown in ASC transfected 293T cells, but not in WT 293T cells. Overexpression validation2287

Field of Research

Signal Transduction

Purification

IKK alpha Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/ml

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (94%), Mouse: (79%)

Buffer

IKK alpha Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

IKK alpha antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

85 kDa

Fragment

IgG

Specificity

Antibody has no cross response to IKKb or IKKg.

Symbol

CHUK

Positive Control 2

Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3

Cat. No. 17-005 - Jurkat Cell Slide

NCBI Official Name

Conserved helix-loop-helix ubiquitous kinase

NCBI Organism

Homo sapiens

Background Reference 01

DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive Iκ B kinase that activates the transcription factor NF-κ B. Nature 1997;388:548-54

Background Reference 02

Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an Iκ B kinase. Cell 1997;90:373-83

Background Reference 03

Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The Iκ B kinase complex (IKK) contains two kinase subunits, IKKα and IKKβ , necessary for Iκ B phosphorylation and NF-κ B activation. Cell 1997;91:243-52

Background Reference 04

Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. Iκ B kinase-β : NF-κ B activation and complex formation with Iκ B kinase-α and NIK. Science 1997;278:866-9

Other Product Names

IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase alpha