STAT1 alpha Antibody

Product Specifications

Background

STAT1 alpha Antibody: STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-α , IFN-β , IFN-γ and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1) .

NCBI Gene ID

6772

Swiss Prot

P42224

Accession Number

NP_009330

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

1021

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar STAT1 alpha expression profile in human cell lines detected by two independent anti-STAT1 alpha antibodies that recognize different epitopes, 1021 against C-terminus domain and competitor antibody against C-terminus domain. STAT1 alpha proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines1021 competitor antibody

Field of Research

Signal Transduction

Purification

STAT1 alpha Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/ml

Homology

Predicted species reactivity based on immunogen sequence: Pig: (94%)

Buffer

STAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

STAT1 alpha antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least two isoforms of STAT1 are known to exist; this antibody will only recognize the larger isoform.

Isoform

Human STAT1 alpha has two isoforms, including isoform 1 (750aa, 87.3kD) and isoform 2 (712aa, 83kD) . Mouse STAT1 alpha has one isoform (749aa, 87.2kD) and Rat STAT1 alpha also has one isoform (749aa, 87.2kD) . 1021 can detect longer human isoform, mouse and rat, but it cannot detect the shorter human isoform.

Symbol

STAT1

Positive Control 2

Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3

Cat. No. 1202 - A431 Cell Lysate

Positive Control 4

Cat. No. 1204 - K562 Cell Lysate

Positive Control 5

Cat. No. 17-001 - HeLa Cell Slide

NCBI Official Name

Signal transducer and activator of transcription 1, 91kDa

NCBI Organism

Homo sapiens

Background Reference 01

Leonard and O'Shea. Annu. Rev. Immunol. 1998; 16:293-322.

Background Reference 02

Schindler and Darnell. Annu. Rev. Biochem. 1995; 64:621-51.

Background Reference 03

Darnell. Science 1997; 277:1630-5.

Background Reference 04

Schindler et al. Proc. Natl. Acad. Sci. USA 1992; 89:7836-9.

Other Product Names

STAT1 alpha Antibody: CANDF7, ISGF-3, STAT91

Tested Applications

ELISA, WB, ICC, IP, IHC-P, IF

Protein ID

6274552

Physical Properties

Liquid

Available Sizes

Curated Selection

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