PRAME (NM_206955) Human Recombinant Protein

Product Specifications

Background

This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Overview

Purified recombinant protein of Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 4

Synonyms

CT130; MAPE; OIP-4; OIP4

Gene ID

23532

UniProt

P78395, A0A024R1E6, B7Z986

Accession Number mRNA

NM_206955

Chromosomal Location

22q11.22

Expression System

HEK293T

Tag

C-Myc/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

57.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.