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HLCS (NM_000411) Human Recombinant Protein

Product Specifications

Background

This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011]

Overview

Recombinant protein of human holocarboxylase synthetase (biotin- (proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing) ) ligase) (HLCS)

Synonyms

HCS

Gene ID

3141

UniProt

P50747

Accession Number mRNA

NM_000411

Chromosomal Location

21q22.13

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Biotin metabolism, Metabolic pathways

Field of Research

Biotin metabolism, Metabolic pathways

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Molecular Weight

80.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

HLCS

Species

Human

Protein ID

NP_000402

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/3141

Uniprot URL

https://www.uniprot.org/uniprot/P50747

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_000411

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_000402

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