Ngef (NM_001111314) Mouse Recombinant Protein
Product Specifications
Background
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.[UniProtKB/Swiss-Prot Function]
Synonyms
Ephexin; Tims2
Gene ID
53972
UniProt
Q8CHT1, E9QK62
Accession Number mRNA
NM_001111314
Chromosomal Location
1 44.42 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
82.2 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Ngef
Species
Mouse
Protein ID
NP_001104784
Overview
Purified recombinant protein of Mouse neuronal guanine nucleotide exchange factor (Ngef), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/53972
Uniprot URL
https://www.uniprot.org/uniprot/Q8CHT1
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_001111314
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_001104784
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