Hectd3 (NM_175244) Mouse Recombinant Protein

Product Specifications

Background

E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus facilitating cell cycle progression by regulating the turn-over of TRIOBP (By similarity) . Mediates also ubiquitination of STX8.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse HECT domain E3 ubiquitin protein ligase 3 (Hectd3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

1700064K09Rik; AI467540; AW743312

Gene ID

76608

UniProt

Q3U487

Accession Number mRNA

NM_175244

Chromosomal Location

4 D1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

97.3 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.