OX40L/TNFSF4 Protein, Human, Recombinant (ECD, mFc Tag)

Product Specifications

Background

OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX40. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig- and CD4-stimulated B cells, this cytokine along with CD7 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.

Overview

A DNA sequence encoding the human TNFSF4 (NP_003317.1) (Gln51-Leu183) was expressed with the Fc region of mouse IgG1 at the N-terminus.

Synonyms

CD134L Protein, Human; CD252 Protein, Human; GP34 Protein, Human; OX-40L Protein, Human; OX4OL Protein, Human; TXGP1 Protein, Human

Gene Name

Tumor necrosis factor (ligand) superfamily, member 4

Expression System

HEK293 Cells

Tag

N-mFc

Field of Research

Cancer Drug Targets

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA.Immobilized recombinant Human TNFRSF4-His (Cat:PH50056M2) at 10 μg/ml (100 μl/well) can bind human S4-mFc3-TNFSF4 (Cat:PH52200M3) with a linear range of 1.28-20 μg/ml.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

TNF Superfamily proteins, CD Molecule proteins, Cancer proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins, Cytokine Proteins, Growth Factor proteins

Molecular Weight

The recombinant human TNFSF4 consists of 369 amino acids and predicts a molecular mass of 42.1 kDa. As a result of glycosylation, it migrates as an approximately 53.3 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.