TNFR-1/CD120a Protein, Human, Recombinant (His Tag)

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD) . CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

CD120a Protein, Human; FPF Protein, Human; MS5 Protein, Human; p55 Protein, Human; p55-R Protein, Human; p60 Protein, Human; TBP1 Protein, Human; TNF-R Protein, Human; TNF-R-I Protein, Human; TNF-R55 Protein, Human; TNFAR Protein, Human; TNFR1 Protein, Human; TNFR1-d2 Protein, Human; TNFR55 Protein, Human; TNFR60 Protein, Human

1. Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) can bind biotinylated human TNFα. The ECof biotinylated human TNFα is 5-12 μg/mL. 2. Measured by its ability to inhibit TNFα-mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The EDfor this effect is typically 0.05-0.4μg/mL in the presence of 0.25 ng/mL recombinant human TNFα.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant human TNFRI consists of 201 amino acids after removal of the signal peptide and has a predicted molecular mass of 22.7 kDa. As a result of glycosylation, the apparent molecular mass of rh TNFRI is 30-35 kDa in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.