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Pig βEP (Beta-Endorphin) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig βEP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig βEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig βEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

Beta EP; b-EP

Gene Aliases

Beta EP; b-EP

Reactivity

Pig

Field of Research

Signal transduction, Endocrinology, Neuro science, Hormone metabolism

Assay Type

Competitive Inhibition

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig βEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig βEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig βEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

2.5 H

Standard

1000 pg/mL

Sample Type

Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Detection Range

15.63 - 1000 pg/mL

Sensitivity

4.95 pg/mL

Product Datasheet

https://www.elkbiotech.com/upload/file/ELISA/ELK0332-1.pdf

Species

Pig

CAS Number

7732-18-5

Available Sizes

Curated Selection

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