Pig βEP (Beta-Endorphin) ELISA Kit
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig βEP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig βEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig βEP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
Beta EP; b-EP
Gene Aliases
Beta EP; b-EP
Reactivity
Pig
Field of Research
Signal transduction, Endocrinology, Neuro science, Hormone metabolism
Assay Type
Competitive Inhibition
Assay Principle
Assay Performance Time
2.5 H
Standard
1000 pg/mL
Sample Type
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.63 - 1000 pg/mL
Sensitivity
4.95 pg/mL
Product Datasheet
https://www.elkbiotech.com/upload/file/ELISA/ELK0332-1.pdf
Species
Pig
CAS Number
7732-18-5
Available Sizes
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