Mini Samples ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Oxidized Low Density Lipoprotein (OxLDL) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Oxidized Low Density Lipoprotein (OxLDL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Oxidized Low Density Lipoprotein (OxLDL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Oxidized Low Density Lipoprotein (OxLDL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

1. Prepare all reagents, samples and standards; <br/> 2. Add 25&micro; L standard or sample to each well. Incubate 1 hour at 37&deg; C; <br/> 3. Aspirate and add 25&micro; L prepared Detection Reagent A. Incubate 1 hour at 37&deg; C; <br/> 4. Aspirate and wash 3 times; <br/> 5. Add 25&micro; L prepared Detection Reagent B. Incubate 30 minutes at 37&deg; C; <br/> 6. Aspirate and wash 5 times; <br/> 7. Add 25&micro; L Substrate Solution. Incubate 10-20 minutes at 37&deg; C; <br/> 8. Add 20&micro; L Stop Solution. Read at 450nm immediately.<br/>

<p> Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Mini Samples Oxidized Low Density Lipoprotein (OxLDL) were tested 20 times on one plate, respectively. <br/> Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Mini Samples Oxidized Low Density Lipoprotein (OxLDL) were tested on 3 different plates, 8 replicates in each plate. <br/> CV (%) = SD/meanX100 <br/> Intra-Assay: CV<10% <br/> Inter-Assay: CV<12% <br/> </p>

<p> The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. <br/> To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. </p>

<p> This assay has high sensitivity and excellent specificity for detection of Mini Samples Oxidized Low Density Lipoprotein (OxLDL) .<br/> No significant cross-reactivity or interference between Mini Samples Oxidized Low Density Lipoprotein (OxLDL) and analogues was observed. </p>