Hoechst 33342
Hoechst 33342 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].
Product Specifications
CAS Number
[23491-52-3]
Product Name Alternative
BisBenzimide H 33342; HOE 33342
UNSPSC
12171500
Hazard Statement
H302, H315, H335, H341
Target
Autophagy
Type
Dye Reagents
Related Pathways
Autophagy
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/hoechst-33342.html
Purity
99.52
Solubility
DMSO : 10 mg/mL (ultrasonic) |H2O : < 0.1 mg/mL
Smiles
CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1
Molecular Formula
C27H28N6O
Molecular Weight
452.55
Precautions
H302, H315, H335, H341
References & Citations
[1]Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011 (1) :pdb.prot5557.
Shipping Conditions
Room Temperature
Storage Conditions
4°C (Powder, protect from light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported
Citation 01
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