Thymidine

Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication[1][2][3].

Product Specifications

CAS Number

[50-89-5]

Product Name Alternative

DThyd; NSC 21548

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

DNA/RNA Synthesis; Endogenous Metabolite; Orthopoxvirus

Type

Natural Products

Related Pathways

Anti-infection; Cell Cycle/DNA Damage; Metabolic Enzyme/Protease

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/Thymidine.html

Purity

99.96

Solubility

DMSO : 50 mg/mL (ultrasonic) |H2O : 33.33 mg/mL (ultrasonic)

Smiles

O[C@H]1C[C@H](N2C(NC(C(C)=C2)=O)=O)O[C@@H]1CO

Molecular Formula

C10H14N2O5

Molecular Weight

242.23

Precautions

H302, H315, H319, H335

References & Citations

[1]Chen G, et al. Cell Synchronization by Double Thymidine Block. Bio Protoc. 2018 Sep 5;8 (17) .|[2]FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181 (4604) :274-5.FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181 (4604) :274-5.|[3]Izeradjene K, et al. Inhibition of thymidine synthesis by folate analogues induces a Fas-Fas ligand-independentdeletion of superantigen-reactive peripheral T cells. Int Immunol. 2001 Jan;13 (1) :85-93.