Murashige & Skoog (MS) Basal Medium w/ Vitamins

How To Use Murashige & Skoog Media Follow the given steps to prepare 1 liter of tissue culture media using MS media: Take a beaker and add 800 ml of distilled water to it. To the water add PCT’s MS Media 4.54 grams, 30 grams of sugar, and PPM™ (generally 1-2 ml/L) Make up the volume of the media to 1000ml. Adjust the pH of the media between 5.4-5.8. Add gelling agent (3-4 g/L gellan gum or 6-8 g/L agar) to the media and keep stirring. Autoclave the medium for 20 minutes at 15 psi and 121°C. Your media is ready! Note: Media should be prepared according to the formula mentioned on the label. However, it is recommended to use an entire container at once. Heat-liable substrates should be added, after autoclaving.

Easy to use Supply nutrients required by in vitro plants for their proper growth and development Provides a well-balanced composition of nutrients, including essential minerals and vitamins, supporting the growth of various plant tissues. Effective in inducing callus formation A dynamic supplier of numerous amino acids, organic acids, plant growth regulators (such as cytokinins and auxins), vitamins, sugars, minerals, nucleic acids, and unidentified growth-promoting substances.