TMA-DPH

TMA-DPH is a hydrophobic fluorescent membrane probe (Ex=355 nm; Em=430 nm) . TMA-DPH is able to anchor on the cell surface and localize to different regions of the phospholipid bilayer. By analyzing the fluorescence polarization values of TMA-DPH in the plasma membrane and membrane substructures, the fluidity of the cell membrane can be determined[1][2][3].

Product Specifications

CAS Number

[115534-33-3]

UNSPSC

12171500

Hazard Statement

H302, H315, H319

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/TMA-DPH.html

Purity

99.86

Solubility

DMSO : 10 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

C[N+](C)(C)C1=CC=C(/C=C/C=C/C=C/C2=CC=CC=C2)C=C1.[O-]S(=O)(C3=CC=C(C)C=C3)=O

Molecular Formula

C28H31NO3S

Molecular Weight

461.62

Precautions

H302, H315, H319

References & Citations

[1]Illinger D, et al. The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells. J Cell Biol. 1994 May;125 (4) :783-94.|[2]Illinger D, et al. A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane. Biochim Biophys Acta. 1995 Oct 4;1239 (1) :58-66.|[3]Benedetti A, et al. Plasma membrane fluidity in isolated rat hepatocytes: comparative study using DPH and TMA-DPH as fluorescent probes. J Gastroenterol Hepatol. 1989 May-Jun;4 (3) :221-7.