Anti-GIT1 Antibody

Mouse Monoclonal Antibody specific to GIT1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-GIT1 Mouse Monoclonal Antibody

Gene Name

Git1

NCBI Gene ID

UniProt

Q9Z272.1

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 375-770 (C-terminus) of rat GIT1 (accession no.Q9Z272).

Target Antigen

ARF GTPase-activating protein GIT1

Target

GIT1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IP

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~90kDa. Positive control: rat brain lysate. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse and rat GIT1. It does not cross-react with GIT2.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains for small GTPases, kinases, the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT1 is localized to focal adhesions, cyto- plasmic complexes and membrane protrusions, and it regulates cell protrusion formation and cell migration.