Colchicine

Colchicine, an orally active alkaloid, is a potent tubulin inhibitor and a microtubule disrupting agent. Colchicine inhibits microtubule polymerization with an IC50 of 3 nM. Colchicine is also a competitive antagonist of the α3 glycine receptors (GlyRs) . Colchicine prevents non-steroidal anti-inflammatory drug (NSAID) -induced small intestinal injury by inhibiting activation of the NLRP3 inflammasome. Colchicine has extensive anti-inflammatory, immunosuppressive and strong anti-fibrosis effects and has the potential for gouty arthritis research[1][2][3][4][5].

Product Specifications

CAS Number

[64-86-8]

UNSPSC

12352005

Hazard Statement

H300, H340

Target

Apoptosis; Autophagy; Microtubule/Tubulin; NOD-like Receptor (NLR)

Type

Natural Products

Related Pathways

Apoptosis; Autophagy; Cell Cycle/DNA Damage; Cytoskeleton; Immunology/Inflammation

Applications

Cancer-programmed cell death

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/Colchicine.html

Concentration

10mM

Purity

99.98

Solubility

DMSO : 100 mg/mL (ultrasonic) |H2O : ≥ 33.33 mg/mL

Smiles

CC(N[C@H](C1=C2)CCC3=CC(OC)=C(OC)C(OC)=C3C1=CC=C(OC)C2=O)=O

Molecular Formula

C22H25NO6

Molecular Weight

399.44

Precautions

H300, H340

References & Citations

[1]Bonfoco E, et al. Colchicine induces apoptosis in cerebellar granule cells. Exp Cell Res. 1995 May;218 (1) :189-200.|[2]Hastie SB. Interactions of colchicine with tubulin. Pharmacol Ther. 1991;51 (3) :377-401|[3]Otani K, et al. Colchicine prevents NSAID-induced small intestinal injury by inhibiting activation of the NLRP3 inflammasome. Sci Rep. 2016 Sep 2;6:32587.|[4]Carola Muñoz-Montesino, et al. Inhibition of the Glycine Receptor alpha 3 Function by Colchicine. Front Pharmacol. 2020 Jul 30;11:1143.|[5]Yuanjun Tang, et al. Network Pharmacology-Based Investigation and Experimental Exploration of the Antiapoptotic Mechanism of Colchicine on Myocardial Ischemia Reperfusion Injury. Front Pharmacol. 2021 Dec 16:12:804030. |[6]Sami H Hammadi, et al. Effect of sacubitril/valsartan on cognitive impairment in colchicine-induced Alzheimer's model in rats. Fundam Clin Pharmacol. 2023 Apr;37 (2) :275-286.