Anti-Calnexin Antibody

Background

Calnexin is a vital chaperone protein involved in the quality control of glycoprotein folding within the endoplasmic reticulum (ER), facilitating correct folding and preventing aggregation. Calnexin’s calcium-dependent binding highlights its role in calcium homeostasis within the ER. The importance of calnexin extends beyond protein folding; it is crucial for immune function, as it assists in the assembly of major histocompatibility complex (MHC) class I molecules, thus playing a part in antigen presentation. Defects or dysregulation in calnexin prominently feature in cystic fibrosis (Pind et al. 1994), where misfolding of the CFTR glycoprotein is not efficiently managed by the calnexin cycle, leading to its degradation and resulting in disease. Due to its importance in protein folding and homeostasis, calnexin dysfunction can also lead to neurodegenerative disorders including Alzheimer’s, Parkinson’s, ALS, and Huntington’s. Dysregulation of these processes can also contribute to tumor development and progression, making calnexin a critical player in cancers including breast, prostate, liver, and lung. Recent studies have identified calnexin as a dual-role biomarker in lung cancer by utilizing anti-Calnexin antibodies for both diagnostic and therapeutic targeting (Lim et al. 2024) .

Short Description

Our Anti-CANX (Calnexin) chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat CANX (Calnexin), and is affinity-purified IgY. It is great for use in IHC, ICC, WB.

Synonyms

Calnexin, IP90, P90, CNX, Major Histocompatibility Complex Class I Antigen-Binding Protein P88, Epididymis Secretory Sperm Binding Protein

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

CANX

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic peptide

Immunogen Type

Human, Mouse, Rat

Target

Calnexin

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

ICC, IHC, WB

Field of Research

Neurodegenerative Disease Alzheimer's Disease Parkinson's Disease Amyotrophic Lateral Sclerosis (ALS) Huntington's Disease Oncoproteins

Assay Type

Primary Antibody

Assay Protocol

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Concentration

200 µg/mL

Format

Affinity-Purified IgY

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

90 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Product Datasheet

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/CANX_datasheet.pdf

IHC Dilution

1:100-1:500

WB Dilution

1:2000-1:10000

Specificity

No cross-reactivity reported

Pre Treatment

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Target Description

Calnexin is a vital chaperone protein involved in the quality control of glycoprotein folding within the endoplasmic reticulum (ER), facilitating correct folding and preventing aggregation. Calnexin’s calcium-dependent binding highlights its role in calcium homeostasis within the ER. The importance of calnexin extends beyond protein folding; it is crucial for immune function, as it assists in the assembly of major histocompatibility complex (MHC) class I molecules, thus playing a part in antigen presentation. Defects or dysregulation in calnexin prominently feature in cystic fibrosis (Pind et al. 1994), where misfolding of the CFTR glycoprotein is not efficiently managed by the calnexin cycle, leading to its degradation and resulting in disease. Due to its importance in protein folding and homeostasis, calnexin dysfunction can also lead to neurodegenerative disorders including Alzheimer’s, Parkinson’s, ALS, and Huntington’s. Dysregulation of these processes can also contribute to tumor development and progression, making calnexin a critical player in cancers including breast, prostate, liver, and lung. Recent studies have identified calnexin as a dual-role biomarker in lung cancer by utilizing anti-Calnexin antibodies for both diagnostic and therapeutic targeting (Lim et al. 2024).

ICC Dilution

1:100-1:500

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe HeLa cell lysate.

Brid

AB_3105808

Physical Properties

Liquid

Isotype

IgY

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Anti-Calnexin Antibody

Product Specifications

Background

Short Description

Synonyms

Other Statements

Gene Name

Host

Species Reactivity

Reactivity

Immunogen

Immunogen Type

Target

Clonality

Isotype

Conjugation

Applications

Field of Research

Assay Type

Assay Protocol

Concentration

Format

Form

Buffer

Molecular Weight

Precautions

Shipping Conditions

Storage Conditions

Shelf Life

Product Datasheet

IHC Dilution

WB Dilution

Specificity

Pre Treatment

Target Description

ICC Dilution

Quality Control

Brid

Physical Properties

Isotype

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