Anti-FITC | fluorescein isothiocyanate, HRP-conjugated (mono
Product Specifications
Background
FITCis a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.
Host
Mouse
Reactivity
FITC molecule
Immunogen
Highly purified fluorescein isothiocyanate isomer 1
Clonality
Monoclonal
Applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC) paraffin, Dot blot (Dot), Western blot (WB)
Dilution
The optimum working dilution should be determined by titration by end user.1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).
Purity
Purified IgG.1 in PBS pH 7.2, HRP conjugated.
Format
Lyophilized
Reconstitution
For reconstitution add 0.5 ml of sterile water
Precautions
This product has not been tested for cross-reactivity with other fluorescent compounds
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc67041a/datasheet/as18 4215.pdf
CAS Number
9007-83-4
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