Anti-Clathrin heavy-chain 1,2

Clathrinis a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.

KLH-conjugated peptide derived from available plant clathrin heavy chain sequences includingArabidopsis thalianaclathrin heavy chain 1 UniProt:Q0WNJ6, TAIR:At3g11130,clathrin heavy chain 2 UniProt:Q0WLB5,TAIR:At3g08530

Takáčet al. (2024). Actin cytoskeleton and plasma membrane aquaporins are involved in different drought response of Arabidopsis rhd2 and der1 root hair mutants. Plant Physiology and Biochemistry Available online 19 September 2024, 109137.Suannoet al. (2023) Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX, Front. Plant Sci., 25 January 2023, Sec. Plant Membrane Traffic and Transport, Volume 14 - 2023, https://doi.org/10.3389/fpls.2023.1090026Panet al. (2023). Stress-induced endocytosis from chloroplast inner envelope membrane is mediated by CHLOROPLAST VESICULATION but inhibited by GAPC.Cell Rep. 2023 Oct 31;42(10):113208. doi: 10.1016/j.celrep.2023.113208.Fujimotoet al. (2020) Longin R-SNARE is retrieved from the plasma membrane by ANTH domain-containing proteins in Arabidopsis. Proc Natl Acad Sci U S A. 2020 Oct 6;117(40):25150-25158. doi: 10.1073/pnas.2011152117. Epub 2020 Sep 23. PMID: 32968023; PMCID: PMC7547277.Ranjanet al. (2019). Transient Internalization and Microtubule-Dependent Trafficking of a Ciliary Signaling Receptor from the Plasma Membrane to the Cilium. Curr Biol. 2019 Aug 2. pii: S0960-9822(19)30867-X. doi: 10.1016/j.cub.2019.07.022.Wattelet-Boyeret al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788.Derbyshireet al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.Groneset al. (2015). Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. J Exp Bot. 2015 Apr 28. pii: erv177.McLoughlinet al. (2013). Identification of novel candidate phosphatidic acid binding proteins involved in the salt stress response of Arabidopsis thaliana roots. Biochem J. Jan 17.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.