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Anti-Clathrin heavy-chain 1,2

Product Specifications

Background

Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

820284AS07 260

UniProt

Q0WNJ6

Host

Rabbit

Reactivity

Actinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacumAS07 260

Not reactive in

Nicotiana benthamiana

Immunogen

KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5, TAIR:At3g08530

Clonality

Polyclonal

Applications

Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)

Field of Research

Zea mays

Dilution

1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

193 | 170 kDa (Arabidopsis thaliana)

Precautions

Please, note that fresh samples will provide better restuls (see image below)

References & Citations

Takáč et al. (2024) . Actin cytoskeleton and plasma membrane aquaporins are involved in different drought response of Arabidopsis rhd2 and der1 root hair mutants. Plant Physiology and Biochemistry Available online 19 September 2024, 109137.Pan et al. (2023) . Stress-induced endocytosis from chloroplast inner envelope membrane is mediated by CHLOROPLAST VESICULATION but inhibited by GAPC.Cell Rep. 2023 Oct 31;42 (10) :113208. doi: 10.1016/j.celrep.2023.113208.Fujimoto et al. (2020) Longin R-SNARE is retrieved from the plasma membrane by ANTH domain-containing proteins in Arabidopsis. Proc Natl Acad Sci U S A. 2020 Oct 6;117 (40) :25150-25158. doi: 10.1073/pnas.2011152117. Epub 2020 Sep 23. PMID: 32968023; PMCID: PMC7547277.Ranjan et al. (2019) . Transient Internalization and Microtubule-Dependent Trafficking of a Ciliary Signaling Receptor from the Plasma Membrane to the Cilium. Curr Biol. 2019 Aug 2. pii: S0960-9822 (19) 30867-X. doi: 10.1016/j.cub.2019.07.022.Wattelet-Boyer et al. (2016) . Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788.Derbyshire et al. (2015) . Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.Grones et al. (2015) . Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. J Exp Bot. 2015 Apr 28. pii: erv177.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Populus trichocarpa, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays.Species of your interest not listed? Contact us
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