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Anti-AOX1/2 | Plant alternative oxidase 1 and 2

Product Specifications

Background

Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.

Host

Rabbit

Reactivity

Arabidopsis thaliana, Betula nana, Beta vulgaris, Brassica napus, Brassica oleracea, Kandelia candel, Eriphorum vaginatum, Glycine max, Hordeum vulgare, Lupinus luteus, Nicotiana tabacum, Oryza sativa, Picea abies, Pisum sativum, Poa annua, Robinia pseudoacacia, Rosa hybrida,Solanum lycopersicum,Solanum tuberosum, Symplocarpus renifolius, Physcomitrium patens, Tigriopus californicus, Triticum aestivum, Zea mays

Not reactive in

Candidia albicans, Chlamydomonas reinhardtii(use  an antibody to algal AOX1,AS06 152),Stomolophussp2

Immunogen

KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms includingArabidopsis thalianaAOX1A. UniProt:Q39219,TAIR:At3g22370,AOX1B UniProt:O23913, TAIR:AT3G22360,AOX1C UniProt:O22048,TAIR:AT3G27620,and AOX2, UniProt:O22049,TAIR:AT5G64210, Solanum lycopersicumUniProt:Q7XBG9, Oryza sativaUniProt:Q7XT33, AOX1D, TAIR:AT1G32350

Clonality

Polyclonal

Applications

Immunolocalization (IL), Western blot (WB)

Dilution

1 : 750 (IL), 1 : 1000 for 10-20 µg of mitochondrial protein/lane detection (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

36-40 | 36-40 forArabidopsis thaliana

Precautions

According toKonertet al. (2015) AOX antibody is recognizing AOX1A and AOX1D.This product can be sold containing ProClin if requested.

References & Citations

Hoaet al. (2024). Proteomic analysis on symbiotic differentiation of mitochondria in soybean nodules. Comparative Study Plant Cell Physiol. 2004 Mar;45(3):300-8. doi: 10.1093/pcp/pch035.Soriaet al. (2024).Functional resilience: An active oxidative phosphorylation system prevails amid foreign proteins in holoparasitic plants. Current Plant Biology Volume 37, March 2024, 100322.Chenet al. (2023) The pentatricopeptide repeat protein EMP601 functions in maize seed development by affecting RNA editing of mitochondrial transcript ccmC, https://doi.org/10.1016/j.cj.2023.03.004.Rodrigueset al. (2023). Germination of Pisum sativum L. Seeds Is Associated with the Alternative Respiratory Pathway. Biology (Basel). 2023 Oct 9;12(10):1318.doi: 10.3390/biology12101318.Wang, Y., Ye, H., Gao, K. et al. The opening of mitochondrial permeability transition pore (mPTP) and the inhibition of electron transfer chain (ETC) induce mitophagy in wheat roots under waterlogging stress. Protoplasma 260, 1179–1191 (2023).Vainonenet al. (2023) Poly(ADP-ribose)-binding protein RCD1 is a plant PARylation reader regulated by Photoregulatory Protein Kinases. Commun Biol. 2023 Apr 19;6(1):429. doi: 10.1038/s42003-023-04794-2Nguyenet al. (2022). MISF2 Encodes an Essential Mitochondrial Splicing Cofactor Required for nad2 mRNA Processing and Embryo Development in Arabidopsis thaliana. Int J Mol Sci. 2022 Feb 28;23(5):2670. doi: 10.3390/ijms23052670. PMID: 35269810; PMCID: PMC8910670.Britoet al. (2022) The role of the electron-transfer flavoprotein: ubiquinone oxidoreductase following carbohydrate starvation in Arabidopsis cell cultures. Plant Cell Rep. 2022 Jan 15. doi: 10.1007/s00299-021-02822-1. Epub ahead of print. PMID: 35031834.Pascualet al (2021). ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response, Plant Physiology, 2021;, kiab225, https://doi.org/10.1093/plphys/kiab225Challabathulaet al. (2021) Differential modulation of photosynthesis, ROS and antioxidant enzyme activities in stress-sensitive and -tolerant rice cultivars during salinity and drought upon restriction of COX and AOX pathways of mitochondrial oxidative electron transport, Journal of Plant Physiology,Volume 268,2022,153583, ISSN 0176-1617,https://doi.org/10.1016/j.jplph.2021.153583.Ohet al. (2021) Alternative oxidase (AOX) 1a and 1d limit proline-induced oxidative stress and aid salinity recovery in Arabidopsis. Plant Physiol. 2021 Dec 17:kiab578. doi: 10.1093/plphys/kiab578. Epub ahead of print. PMID: 34919733.Pavlovic& Kocab. (2021) Alternative oxidase (AOX) in the carnivorous pitcher plants of the genus Nepenthes: what is it good for? Ann Bot. 2021 Dec 18:mcab151. doi: 10.1093/aob/mcab151. Epub ahead of print. PMID: 34922341.Makinoet al. (2020). Induction of Terminal Oxidases of Electron Transport Chain in Broccoli Heads Under Controlled Atmosphere Storage. Foods, 9 (4)Marchettiet al. (2020). Mitochondrial Pentatricopeptide Repeat Protein, EMB2794, Plays a Pivotal Role in NADH Dehydrogenase Subunit nad2 mRNA Maturation in Arabidopsis Thaliana. Plant Cell Physiol DOI: 10.1093/pcp/pcaa028Garmashet al. (2020). Altered levels of AOX1a expression result in changes in metabolic pathways in Arabidopsis thaliana plants acclimated to low dose rates of ultraviolet B radiation. Plant Sci. 2020 Feb;291:110332. doi: 10.1016/j.plantsci.2019.110332.Kuanget al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.Twardet al. (2019). Identification of the alternative oxidase gene and its expression in the copepod Tigriopus californicus. Comp Biochem Physiol B Biochem Mol Biol. 2019 Feb;228:41-50. doi: 10.1016/j.cbpb.2018.11.003.Rethoreet al. (2019). Arabidopsis seedlings display a remarkable resilience under severe mineral starvation using their metabolic plasticity to remain self-sufficient for weeks. Plant J. 2019 Mar 22. doi: 10.1111/tpj.14325.Luevano-Martínezet al. (2019). Mitochondrial alternative oxidase is determinant for growth and sporulation in the early diverging fungus Blastocladiella emersonii. Fungal Biology, Vol 123, Issue 1, 59-65.Cordobaet al. (2019). Different Types of CA Domains Are Present in Complex I from Immature Seeds and Adult Plants in Arabidopsis thaliana. Plant Cell Physiol. 2019 Jan 22. doi: 10.1093/pcp/pcz011.Czoboret al. (2019). Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. PLoS One. 2019 Jan 10;14(1):e0210592. doi: 10.1371/journal.pone.0210592.Huet al. (2018). OsNDUFA9 encoding a mitochondrial complex I subunit is essential for embryo development and starch synthesis in rice. Plant Cell Rep. 2018 Dec;37(12):1667-1679. doi: 10.1007/s00299-018-2338-x.Borovikand Grabelnych (2018). Mitochondrial alternative cyanide-resistant oxidase is involved in an increase of heat stress tolerance in spring wheat. J Plant Physiol. 2018 Dec;231:310-317. doi: 10.1016/j.jplph.2018.10.007.Umekawaand Ito (2018). Thioredoxin o-mediated reduction of mitochondrial alternative oxidase in the thermogenic skunk cabbage Symplocarpus renifolius. J Biochem. 2018 Oct 5. doi: 10.1093/jb/mvy082.Huet al. (2018). OsNDUFA9 encoding a mitochondrial complex I subunit is essential for embryo development and starch synthesis in rice.Plant Cell Rep. 2018 Aug 27. doi: 10.1007/s00299-018-2338-x.Zhuet al. (2018). Mitochondrial alternative oxidase-dependent autophagy involved in ethylene-mediated drought tolerance in Solanum lycopersicum. Plant Biotechnol J. 2018 May 4. doi: 10.1111/pbi.12939.Garmashet al. (2017). Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation. J Plant Physiol. 2017 Aug;215:110-121. doi: 10.1016/j.jplph.2017.05.023.Vishwakarmaet al. (2016). A discrete role for alternative oxidase under hypoxia to increase nitric oxide and drive energy production. Free Radic Biol Med. 2018 Mar 28. pii: S0891-5849(18)30148-5. doi: 10.1016/j.freeradbiomed.2018.03.045.Zhaoet al. (2016). Nitrogen deprivation induces cross-tolerance of Poa annua callus to salt stress. Biologia Plantarum 60 (3): 543–554.Soltiet al. (2016). Does a voltage-sensitive outer envelope transport mechanism contributes to the chloroplast iron uptake? Planta. 2016 Dec;244(6):1303-1313. Epub 2016 Aug 19.Zhanget al. (2016). A High Temperature-Dependent Mitochondrial Lipase EXTRA GLUME1 Promotes Floral Phenotypic Robustness against Temperature Fluctuation in Rice (Oryza sativa L.). PLoS Genet. 2016 Jul 1;12(7):e1006152. doi: 10.1371/journal.pgen.1006152. eCollection 2016.Menget al. (2016). Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.). Sci Rep. 2016 Mar 15;6:23098. doi: 10.1038/srep23098Pavlovicet al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.Konertet al.(2015). Protein phosphatase 2A (PP2A) regulatory subunit B'γ interacts with cytoplasmic ACONITASE 3 and modulates the abundance of AOX1A and AOX1D in Arabidopsis thaliana. New Phytol. 2015 Feb;205(3):1250-63. doi: 10.1111/nph.13097. Epub 2014 Oct 13.Langet al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. 2011 Feb;30(2):205-15.doi: 10.1007/s00299-010-0935-4.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70a994c49e6dc66fac3/datasheet/as04 054.pdf

CAS Number

9007-83-4

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