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Anti-human CD14 Monoclonal Antibody Unconjugated, Flow Validated

Boster Bio Anti-human CD14 Monoclonal Antibody Unconjugated, Flow Validated (Catalog# FC00137) . Tested in Flow Cytometry application (s) . This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.

Product Specifications

Background

CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS) . CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP) . Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

Synonyms

Monocyte differentiation antigen CD14 ; Myeloid cell-specific leucine-rich glycoprotein; CD14; Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form; CD14

Gene Name

CD14

UniProt

P08571

Host

Mouse

Reactivity

Human, Rhesus monkey, Cynomolgus monkey, Baboon

Immunogen

Cultured human peripheral blood monocytes

Clonality

Monoclonal

Clone

Clone: Clone: 26ic

Tissue Specificity

Detected on macrophages (at protein level) (PubMed:1698311) . Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

Applications

Flow Cytometry

Field of Research

Cell Type Markers, Hematopoietic Progenitors, Immunology, Innate Immunity, Mesenchymal Stem Cells, Myeloid, Stem Cells, TLR Signaling

Purification

Protein A purified

Sensitivity

5ul per sample

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

User needs to optimize the dilution ratio for this antibody.

Form

Liquid

Function

Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655) . In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655) . Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135) . Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211) . Binds electronegative LDL (LDL (-) ) and mediates the cytokine release induced by LDL (-) (PubMed:23880187) . .

Molecular Weight

40076 MW

Shipping Conditions

Available

Storage Conditions

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=FC00137

Product MSDS

https://www.bosterbio.com/msds?sku=FC00137

Fragment

IgG2b, k

Other Gene Names

Monocyte differentiation antigen CD14

Subcellular Location

Cell membrane; Lipid- anchor, GPI-anchor. Secreted. Membrane raft. Golgi apparatus. Secreted forms may arise by cleavage of the GPI anchor.

Protein Name

Monocyte differentiation antigen CD14

Available Sizes

Curated Selection

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