Vimentin Human Recombinant Protein
Vimentin Human Recombinant produced in E.coli cells is a single non-glycosylated protein containing 465 amino acids chain and having a molecular mass of 53.5kDa. The Vimentin is purified by proprietary chromatographic techniques.
Product Specifications
Synonyms
Vimentin; Vim; FLJ36605
Gene Name
VIM
UniProt
P08670
Reactivity
Human
Tissue Specificity
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
Source
Escherichia coli
Sequence
[object Object]
Purification
Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Reconstitution
It is recommended to reconstitute the lyophilized Vimentin in sterile 4mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Shipping Conditions
Available
Storage Conditions
Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.
Product Datasheet
https://www.bosterbio.com/datasheet?sku=PROTP08670
Product MSDS
https://www.bosterbio.com/msds?sku=PROTP08670
Other Gene Names
Vimentin
Subcellular Location
Cytoplasm
Available Sizes
Curated Selection
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