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Anti-BCAT1 Antibody Picoband®

Boster Bio Anti-BCAT1 Antibody catalog # PA1538. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.

Synonyms

Branched-chain-amino-acid aminotransferase, cytosolic; BCAT (c) ;2.6.1.42; Protein ECA39; BCAT1; BCT1, ECA39

Gene Name

Branched-chain-amino-acid aminotransferase, cytosolic

UniProt

P54687

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT1, different from the related mouse sequence by one amino acid, rat sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.

Applications

IHC, WB

Field of Research

Amino Acid Metabolism, Amino Acids, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=PA1538

Product MSDS

https://www.bosterbio.com/msds?sku=PA1538

Fragment

Rabbit IgG

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat<br>Western blot, 0.1-0.5μg/ml, Human<br>

Other Gene Names

BCAT1

Subcellular Location

Cytoplasm.

Sequence Similarities

Belongs to the bZIP family.

Protein Name

Branched-chain-amino-acid aminotransferase, cytosolic (BCAT (c) )

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