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Anti-Interleukin-10 IL10 Antibody Picoband®

Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1016. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Synonyms

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10

Gene Name

Interleukin-10

UniProt

P29456

Host

Rabbit

Reactivity

Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived rat IL-10 recombinant protein (Position: S19-N178) .

Clonality

Polyclonal

Tissue Specificity

Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Applications

ELISA, IHC, WB

Field of Research

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=RP1016

Product MSDS

https://www.bosterbio.com/msds?sku=RP1016

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat, By Heat <br>ELISA, 0.1-0.5μg/ml, -<br>

Other Gene Names

IL10

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the IL-10 family.

Protein Name

Interleukin-10 (IL-10)

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