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Anti-EEA1 Antibody Picoband®

Boster Bio Anti-EEA1 Antibody Picoband® catalog # A02296-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

The gene EEA1 encodes for the 1400 amino acid protein, Early Endosome Antigen 1. It localizes exclusively to early endosomes and has an important role in endosomal trafficking. EEA1 binds ly to the phospholipid phosphatidylinositol 3-phosphate through its C-terminal FYVE domain and forms ahomodimer through a coiled coil. Furthermore, EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo.

Synonyms

Early endosome antigen 1; Endosome-associated protein p162; Zinc finger FYVE domain-containing protein 2; EEA1; ZFYVE2

Gene Name

Early endosome antigen 1

UniProt

Q15075

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human EEA1, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Applications

IF, IHC, ICC, WB

Field of Research

Signal Transduction, Subcellular Markers, Tags & Cell Markers, Neuroscience, Protein Trafficking, Organelle Proteins, Organelles, Neurotransmission, Cell Type Marker, Neuron Marker, Intracellular Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=A02296-3

Product MSDS

https://www.bosterbio.com/msds?sku=A02296-3

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml<br> Immunohistochemistry, 2-5 μg/ml<br> Immunocytochemistry/Immunofluorescence, 5μg/ml<br>

Other Gene Names

EEA1

Subcellular Location

Cytoplasm. Early endosome membrane; Peripheral membrane protein.

Protein Name

Arachidonate 12-lipoxygenase, 12S-type

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