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Anti-EGF Antibody Picoband®

Boster Bio Anti-EGF Antibody Picoband® catalog # A00378-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Synonyms

Pro-epidermal growth factor; EGF; Epidermal growth factor; Egf

Gene Name

Pro-epidermal growth factor

UniProt

P07522

Host

Rabbit

Reactivity

Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived rat EGF recombinant protein (Position: N974-R1026) . Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively.

Clonality

Polyclonal

Tissue Specificity

B-cells. Expressed in a number of B-cell lines including P32/ISH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.

Applications

ELISA, IHC, WB

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Growth Factors, Growth Factors/Hormones, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity) . .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=A00378-1

Product MSDS

https://www.bosterbio.com/msds?sku=A00378-1

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat<br> ELISA(Cap) , 1-5μg/ml, Rat<br>

Other Gene Names

Egf

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Cyclic AMP-dependent transcription factor ATF-4

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