Anti-m7GpppN-mRNA hydrolase DCP2 Antibody

The removal, or decapping, of eukaryotic mRNA is an important step in the degradation of mRNA. Decapping protein 2 (DCP2) is the major mRNA decapping enzyme in cells. It is a member of the Nudix hydrolases superfamily of proteins that predominantly catalyze the hydrolysis of small nucleoside diphosphate substrates linked to another moiety. DCP2 is widely expressed in multiple tissues at varying levels, with highest expression seen in testis and brain.

DCP2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DCP2. The immunogen is located within amino acids 340 - 390 of DCP2.

Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed:12417715, PubMed:12218187, PubMed:12923261, PubMed:21070968, PubMed:28002401) . Removes the 7- methyl guanine cap structure from mRNA molecules, yielding a 5'- phosphorylated mRNA fragment and 7m-GDP (PubMed:12486012, PubMed:12923261, PubMed:21070968, PubMed:28002401) . Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (PubMed:14527413) . Plays a role in replication-dependent histone mRNA degradation (PubMed:18172165) . Has higher activity towards mRNAs that lack a poly (A) tail (PubMed:21070968) . Has no activity towards a cap structure lacking an RNA moiety (PubMed:21070968) . The presence of a N (6) - methyladenosine methylation at the second transcribed position of mRNAs (N (6),2'-O-dimethyladenosine cap; m6A (m) ) provides resistance to DCP2-mediated decapping (PubMed:28002401) . Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degration of their transcripts (PubMed:26098573) .

DCP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.