Anti-Proline-rich protein 5 PRR5 Antibody

Proline-rich protein 5 (PRR5), also known as Protor-1, is a 388 amino acid protein in Protor family, is thought to act as a tumor suppressor in breast and colorectal tumorigenesis. PRR5 is widely expressed and possesses two RICTOR interaction sites and a C-terminal Proline rich region. It promotes Rapamycin complex 2 (mTORC2) activity. There are four isoforms of PRR5 that are produced as a result of alternative splicing events and these isoforms play an important role in the modulation of platelet-derived growth factor signaling and in the regulation of PDGFR-beta expression.

PRR5 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human PRR5. The immunogen is located within amino acids 310 - 360 of PRR5.

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. May act as a tumor suppressor in breast cancer.

PRR5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.