Anti-Hormone-sensitive lipase Lipe Antibody

Although initially described as an adipocyte-specific triacylglycerol lipase, Lipe (also known as hormone-specific lipase or HSL) is expressed in multiple tissues and cell lines. It plays multiple roles in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. Lipe is expressed as a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesterol esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Recently, it was observed that the lack of Lipe in genetically obese leptin-null mice inhibited obesity and adipogenesis, suggesting that Lipe plays a major role in adipocyte proliferation.

Lipe antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Lipe. The immunogen is located within amino acids 920 - 970 of Lipe.

Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.

In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

Lipe antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.