Anti-CARMA2 CARD14 Antibody

CARMA proteins belong to the membrane-associated guan-ylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. As with CARMA1, the CARD domain of CARMA2 has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-κB activation. When expressed in cells, this protein activated NF-κB and induced the phosphorylation of BCL10 Alternative splicing of CARMA2 results in isoforms that possess differential effects on NF-κB activation and endoplasmic reticulum stress-induced cell death.

CARMA2 antibody is human specific. At least three isoforms of CARMA2 are known to exist; this antibody will only detect isoform 1. CARMA2 antibody is predicted not to cross-react with other CARMA proteins.

CARMA2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human CARMA2. The immunogen is located within the last 50 amino acids of CARMA2.

Isoform 1 is detected in placenta and epidermal keratinocytes (PubMed:22521418) . Isoform 2 is detected in leukocytes and fetal brain (PubMed:22521418) .

Acts as a scaffolding protein that can activate the inflammatory transcription factor NF-kappa-B and p38/JNK MAP kinase signaling pathways. Forms a signaling complex with BCL10 and MALT1, and activates MALT1 proteolytic activity and inflammatory gene expression. MALT1 is indispensable for CARD14- induced activation of NF-kappa-B and p38/JNK MAP kinases (PubMed:11278692, PubMed:21302310, PubMed:27113748, PubMed:27071417) . May play a role in signaling mediated by TRAF2, TRAF3 and TRAF6 and protects cells against apoptosis.

CARMA2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.