Recombinant Lambda-carrageenase (cglA), partial

Product Specifications

Abbreviation

Recombinant Pseudoalteromonas sp cglA protein, partial

Gene Name

CglA

UniProt

Q05JY7

Expression Region

26-163aa

Organism

Pseudoalteromonas sp

Target Sequence

SQSAIKSIETNRTITKVRTGMLSGGSSIITTSYEGTVAAYKFNGEKLWENELSGFMNHDIWVQDINGDGLVEIFAANADGNVYCINSDGSLKWTFGLNEVPMNSVTVISDADEKYVVAGGYDKNLYYISANGELLKTI

Tag

C-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Hydrolyzes lambda-carrageenan with inversion of anomeric configuration. Does not hydrolyze iota- and kappa-carrageenans, agarose or porphyran.

Endotoxin

Not test

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Not Test

Bioactivity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

21.9 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.