Anti-MITF (phospho-S180) Antibody

Boster Bio Anti-MITF (phospho-S180) Antibody catalog # A00269S180. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32; MITF; BHLHE32;

Gene Name

MITF

UniProt

O75030

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human MITF around the phosphorylation site of Serine 180.

Clonality

Polyclonal

Tissue Specificity

Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE) . Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.

Applications

IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Dilution

IHC: 1:50-1:200

Form

Liquid

Function

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR) . Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) . Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Molecular Weight

58795 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=A00269S180

Product MSDS

https://www.bosterbio.com/msds?sku=A00269S180

Other Gene Names

Microphthalmia-associated transcription factor

Subcellular Location

Nucleus

Protein Name