Anti Human Immunoglobulins (IgG, IgA, IgM) “Polyvalent” FITC

Anti Human Immunoglobulins (IgA, IgA, IgM) "Polyvalent" FITC, Low F/P - 2-4.9 - Direct Tag • Monospecificity by solid phase adsorption for a strong tag with a low background • High antibody titer equals more dilution and dollar value • Choice of F/P ratio; high for indirect tags – low for direct tags • FITC Conjugates • Made in Goat • IN VITRO Diagnostic Use The three major immunoglobulins of serum: IgG, IgA and IgM are used to immunize goats.  Various bleedings are pooled for optimal reactivity with normal sera and for detection of abnormal monoclonal immunoglobulins of each cless.  The gamma globulin fraction of the pooled antisera  is then prepared, followed by reaction with fluorescein isothiocyanate.  The excess dye is removed by dialysis and the conjugated globulin further fractionated on DEAE cellulose to remove under- and overlabelled protein.  Total protein is determined by the Biuret reaction and the concentration of fluorescein by comparison with a fluorescein diacetate standard.  Sodium azide (0.1% w/v) is added as preservative.