ComASP ATM-CZA

ComASP Benzylpenicillin 0.002-32 is not for use directly with clinical or other specimens. The microorganism to be tested must first be isolated on a nonselective culture medium, such as blood agar or tryptic soy agar (TSA). In case of mixed culture, selected colonies should be purified by subculturing. Differential media harboring chromogenic or fluorogenic substrates should not be used for the subculture. It is recommended that cultures be no more than 24 hours old unless additional incubation is required to achieve sufficient growth. Reading the results: At the end of the incubation period observe the growth in the wells and establish the MIC, i.e. the lowest concentration of antibiotic that inhibits visible growth. The result can be read visually or automatically. Reading by the naked eye can be improved by use of bright indirect lighting against a dark background. Growth appears as turbidity or as a button at the bottom of the well (compare with the amount of growth in the growth-control well). The growth-control well should be evaluated first. Make sure it is positive for growth. If not, check the viability of the colonies picked and repeat the test using a new row in the same panel or a new panel and a microbial culture of recent growth. Note the results on the Test Results Form included in the kit (copy as many form as necessary).