HEK293 Host Cell DNA (HEK293 HCD) Residue Detection kit
Purchasing this kit includes a CG-DP100 complimentary Magnetic Residual DNA Sample Preparation Kit-100T, designed for DNA extraction.
Product Specifications
Reproducibility
1) Intra Variation: 10.3 - 12.3 % || 2) Inter Variation: 16 - 19 %
Certification
ISO 13485:2016
Species Reactivity
Human
Target
HEK 293 Host Cell DNA Residue
Applications
PCR
Field of Research
Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from host cell.
Assay Type
PCR
Detection Range
30 fg/μL - 3000 pg/μL, the detection range can also be adjusted.
Precision
1) Intra Variation%: 10.3-12.3 2) Inter Variation%: 16-19
Sensitivity
LOQ: 30fg/μL
Components
(1) 2X qPCR Mix (UDG plus) || (2) Primer&Probe Mix || (3) DNA Dilution Buffer || (4) DNA Control (30ng/μL) || (5) RNase-Free H2O || (6) 50X ROX Reference Dye (Optional)
Storage Conditions
The kit should be stored at -20°C. The shelf life of components B2/B3/B4 is 2 years. The shelf life of components B1/B5/B6 is 1 year.
Species
HEK 293
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