5 (6) -FITC

5 (6) -FITC (Fluorescein 5 (6) -isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5 (6) -FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm[1][2][3][4].

Product Specifications

CAS Number

[27072-45-3]

Product Name Alternative

Fluorescein 5 (6) -isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers

UNSPSC

12171500

Hazard Statement

H302+H332, H315, H317, H319, H334, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/5_6_-FITC.html

Purity

97.59

Solubility

DMSO : ≥ 156 mg/mL

Smiles

OC1=CC=C(C2(O3)C(C=C(N=C=S)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(N=C=S)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6

Molecular Formula

C21H11NO5S

Molecular Weight

389.38

Precautions

H302+H332, H315, H317, H319, H334, H335

References & Citations

[1]J S Miller , ET AL. Flow cytometric identification of microorganisms by dual staining with FITC and PI. Cytometry. 1990;11 (6) :667-75.|[2]S S Twining, et al. Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes.Anal Biochem. 1984 Nov 15;143 (1) :30-4.|[3]K Sugiura, et al. A simple quantitative immunofluorescence assay for FITC-labeled antibody on platelets. J Immunol Methods. 1981;40 (2) :165-70.|[4]Natalia Oddone, et al. Cell uptake mechanisms of PAMAM G4-FITC dendrimer in human myometrial cells. J Nanopart Res. 2013 15:1776.