VEGFR-2/KDR

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs) . All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. Human VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Product Specifications

Synonyms

Vascular endothelial growth factor receptor-2 ; KDR; FLK1; CD309; VEGF receptor 2; VEGFR2; kinase insert domain protein receptor

NCBI Gene ID

3791

UniProt

P35968

Accession Number

NP_002244.1

Accession Number mRNA

NM_002253.2

Chromosomal Location

4q11-q12

Reactivity

Anti-Human

Cross Reactivity

Human

Target Antigen

Recombinant human soluble extracellular KDR (D7)

Clone

(#EWC)

Applications

ELISA, WB, FC

Purification Method

Protein G chromatography

Assay Protocol

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Bioactivity

ELISA: Use at 1-10 µg/ml. Western blotting: Use at 2-5 µg/ml. FACS analysis: Use at 5 µg/ml.

Form

Lyophilized

Buffer

PBS

Reconstitution

Water

Storage Conditions

The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.