TFF-1

The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF1 is essential for normal differentiation of the antral and pyloric gastric mucosa, and functions as a gastric-specific tumor suppressor gene. Recombinant Murine TFF-1 is a 7.4 kDa monomeric protein consisting of a 66 amino acid polypeptide chain, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Product Specifications

Synonyms

Trefoil Factor 1, pS2 protein, HP1.A, Breast Cancer Estrogen-Inducible protein (BCEI), PNR2

NCBI Gene ID

21784

UniProt

Q08423

Accession Number

NP_033388.1

Accession Number mRNA

NM_009362.2

Reactivity

Mouse

Cross Reactivity

Mouse

Sequence

QAQAQAQAQE ETCIMAPRER INCGFPGVTA QQCTERGCCF DDSVRGFPWC FHPMAIENTQ EEECPF

Assay Protocol

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analyses

Bioactivity

Not available!

Length

Form

Lyophilized

Reconstitution

Water

Molecular Weight

7.4 kDa

Storage Conditions

The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.