SRY

The „Sex determining region of Y-Gen” (SRY), known as SRY gene, is coding for a transkription factor (TDF for Testis-determining factor) . It belongs to the protein family of DNA binding-proteins. The SRY gene is beside other genes used for the determination of the sex in human as well as other mammals. Most mammals have for this purpose a further gene, UBE1. In humans SRY is normally located on the short arm of the Y chromosome. Accordingly, people who possess this chromosome with the corresponding gene, normally have a male phenotype. It does not matter how many copies of the X chromosome are present, even people with a multiple X chromosome (Klinefelter's syndrome) have this. The protein encoded by the gene Testis-determining factor controls the development of male sex

Product Specifications

Synonyms

Sex-determining region Y protein; Testis-determining factor

NCBI Gene ID

280931

UniProt

Q03255

Accession Number

NP_001014407.1

Accession Number mRNA

NM_001014385.1

Reactivity

Bovine

Cross Reactivity

Bovine

Sequence

MFRVLNDDVYSPAVVQQQTTLAFRKDSSLCTDSHSANDQCERGEHVRESSQDHVKRPMNAFIVWSRERRRKVALENPKMKNSDISKQLGYEWKRLTDAEKRPFFEEAQRLLAIHRDKYPGYKYRPRRRAKRPQKSLPADSSILCNPMHVETLHPFTYRDGCAKTTYSQMESQLSRSQSVIITNSLLQKEHHSSWTSLGHNKVTLATRISADFPCNKSLEPGLSCAYFQYLE

Assay Protocol

Centrifuge vial prior to opening. Bovine SRY should be reconstituted in PBS to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffered solutions.

Purity

> 90% by SDS-PAGE

Bioactivity

Data not available.

Length

231

Form

Lyophilized

Buffer

Water

Reconstitution

PBS

Molecular Weight

26.8 kDa

Storage Conditions

The lyophilized powder although stable at room temperature for 3 weeks, is best stored desiccated at -20°C. Reconstituted bo SRY should be stored in working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .