MCP-4

Human MCP-4 or CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature MPC-4. Mature MPC-4 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human MCP-4 is most homologous to MCP1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. The mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. MPC-4 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR2 and CCR3, both of which have been shown to bind CCL13.

Product Specifications

Synonyms

CCL13; NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13

NCBI Gene ID

6357

UniProt

Q99616

Accession Number

NP_005399

Accession Number mRNA

NM_005408

Chromosomal Location

17q11.2

Reactivity

Anti-Human

Cross Reactivity

Human

Target Antigen

Recombinant Human MCP-4

Clone

Rabbit IgG

Applications

ELISA, WB, N

Purification Method

Antigen-affinity purified

Assay Protocol

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Form

Lyophilized from PBS

Buffer

PBS

Reconstitution

Water

Storage Conditions

The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.