FnCas9 Protein (Highly Pure from Francisella Novicida)

<strong>FnCas9 Protein (Highly Pure)</strong>_x000D_ <strong>Catalog number:</strong> B2010492_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 50 ug_x000D_ <strong>Molecular Weight or Concentration:</strong> 190 kDa_x000D_ <strong>Supplied as:</strong> Lyophilized powder_x000D_ <strong>Applications:</strong> a tool for plasmid and large genomic DNA fragment cloning_x000D_ <strong>Storage:</strong> −20°C_x000D_ <strong>Keywords:</strong> Francisella Novicida Cas9_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Islam KU, Iqbal J. An Update on Molecular Diagnostics for COVID-19. Front_x000D_ Cell Infect Microbiol. 2020 Nov 10;10:560616._x000D_ _x000D_ 2: Green JC, Hu JS. Editing plants for virus resistance using CRISPR-Cas. Acta_x000D_ Virol. 2017;61(2):138-142._x000D_ _x000D_ 3: Hirano H, Gootenberg JS, Horii T, Abudayyeh OO, Kimura M, Hsu PD, Nakane T,_x000D_ Ishitani R, Hatada I, Zhang F, Nishimasu H, Nureki O. Structure and Engineering_x000D_ of Francisella novicida Cas9. Cell. 2016 Feb 25;164(5):950-61._x000D_ _x000D_ 4: Kumar M, Gulati S, Ansari AH, Phutela R, Acharya S, Azhar M, Murthy J,_x000D_ Kathpalia P, Kanakan A, Maurya R, Vasudevan JS, S A, Pandey R, Maiti S,_x000D_ Chakraborty D. FnCas9-based CRISPR diagnostic for rapid and accurate detection_x000D_ of major SARS-CoV-2 variants on a paper strip. Elife. 2021 Jun 9;10:e67130._x000D_ _x000D_ 5: Azhar M, Phutela R, Kumar M, Ansari AH, Rauthan R, Gulati S, Sharma N, Sinha_x000D_ D, Sharma S, Singh S, Acharya S, Sarkar S, Paul D, Kathpalia P, Aich M, Sehgal_x000D_ P, Ranjan G, Bhoyar RC; Indian CoV2 Genomics &amp; Genetic Epidemiology (IndiCovGEN)_x000D_ Consortium, Singhal K, Lad H, Patra PK, Makharia G, Chandak GR, Pesala B,_x000D_ Chakraborty D, Maiti S. Rapid and accurate nucleobase detection using FnCas9 and_x000D_ its application in COVID-19 diagnosis. Biosens Bioelectron. 2021 Jul_x000D_ 1;183:113207._x000D_ _x000D_ 6: Price AA, Sampson TR, Ratner HK, Grakoui A, Weiss DS. Cas9-mediated targeting_x000D_ of viral RNA in eukaryotic cells. Proc Natl Acad Sci U S A. 2015 May_x000D_ 12;112(19):6164-9._x000D_ _x000D_ 7: Mekler V, Kuznedelov K, Severinov K. Quantification of the affinities of_x000D_ CRISPR-Cas9 nucleases for cognate protospacer adjacent motif (PAM) sequences. J_x000D_ Biol Chem. 2020 May 8;295(19):6509-6517._x000D_ _x000D_ 8: Acharya S, Mishra A, Paul D, Ansari AH, Azhar M, Kumar M, Rauthan R, Sharma_x000D_ N, Aich M, Sinha D, Sharma S, Jain S, Ray A, Jain S, Ramalingam S, Maiti S,_x000D_ Chakraborty D. <i>Francisella novicida</i> Cas9 interrogates genomic DNA with_x000D_ very high specificity and can be used for mammalian genome editing. Proc Natl_x000D_ Acad Sci U S A. 2019 Oct 15;116(42):20959-20968._x000D_ _x000D_ 9: Chen F, Ding X, Feng Y, Seebeck T, Jiang Y, Davis GD. Targeted activation of_x000D_ diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR_x000D_ targeting. Nat Commun. 2017 Apr 7;8:14958._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/29327438/">10: Zhang T, Zheng Q, Yi X, An H, Zhao Y, Ma S, Zhou G. Establishing RNA virus_x000D_ resistance in plants by harnessing CRISPR immune system. Plant Biotechnol J._x000D_ 2018 Aug;16(8):1415-1423. </a>_x000D_ _x000D_ <strong>Products Related to FnCas9 Protein (Highly Pure)</strong> <a href="https://moleculardepot.com/product-category/Proteins/">: Proteins</a>