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Anti-Cytokeratin 7(KRT7/903), CF740 conjugate

Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7 ) from colon carcinomas (keratin 7-).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT7

Gene ID

3855

NCBI Gene ID

411501

UniProt

P08729

Cellular Locus

Cytoskeleton

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant full-length human KRT7 protein

Target Antigen

Cytokeratin 7

Clonality

Monoclonal

Isotype

IgG1

Clone

KRT7/903

Conjugation

CF740

Disease

Tumor

Source

Animal

Applications

Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified)

Validated Applications

FC,IF,IHC,FFPE

Field of Research

Cancer, Cytoskeleton

Positive Control

A431, HeLa cells, Breast cancer

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

54 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 1-2 ug/mL|Does not react with mouse, rat, ferret or rabbit; others not known|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/11643272/datasheet/bnc740903-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/11643272/msds/bnc740903-100.pdf

CAS Number

9007-83-4

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