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Anti-PCNA(PC10), CF740 conjugate

Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

PCNA

Gene ID

5111

NCBI Gene ID

147433 & 728886

UniProt

P12004

Cellular Locus

Nucleus & cytoplasm

Host

Mouse

Species Reactivity

Chicken,Drosophila,Human,Monkey,Mouse,Pig,Rat,Yeaste,Yeast,Zebrafish

Immunogen

Rat PCNA/Protein A fusion protein

Target Antigen

PCNA

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

PC10

Conjugation

CF740

Source

Animal

Applications

Flow, intracellular (verified) | IHC, FFPE (verified)

Validated Applications

FC,IHC,FFPE

Field of Research

Cell cycle

Positive Control

Tonsil or reactive lymph node

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

36 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/11642694/datasheet/bnc740467-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/11642694/msds/bnc740467-500.pdf

CAS Number

9007-83-4

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