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Anti-CD104(UM-A9), CF740 conjugate

CD104 is beta 4 integrin. It is primarily expressed in epithelial cells. Together with alpha 6 integrin, it is the cell surface receptor for laminin, where it mediates cell adhesion. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

CD104; Integrin beta 4; ITGB4; GP150

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ITGB4

Gene ID

3691

NCBI Gene ID

632226

UniProt

P16144

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

Human squamous cell carcinoma (UM-SCC1)

Target Antigen

CD104 | integrin beta 4

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

UM-A9

Conjugation

CF740

Source

Animal

Applications

Flow, surface (published) | Functional studies (published) | IF (published) | IP (published)

Validated Applications

FC,IF,IP

Field of Research

Cancer, Immunology

Positive Control

A431 cells, squamous cell carcinoma, colon or placenta

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

205 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Biological blockade partially blocks laminin binding|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Res (1991) 51(9):2395-402. (IP, functional studies) Cancer Res (1997) 57(1), 38-42. (IP, Flow, IF)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/11642673/datasheet/bnc740449-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/11642673/msds/bnc740449-100.pdf

CAS Number

9007-83-4

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