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Anti-Human IgM Immunoglobulin(IM373), CF740 conjugate

Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

AGM1; IGHM; Constant Region of Heavy Chain of IgM; Ig Mu Chain C Region

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

IGHM

Gene ID

3507

NCBI Gene ID

510635

UniProt

P01871; P20769

Cellular Locus

Plasma membrane|Secreted (extracellular)

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant full-length heavy chain of human IgM

Target Antigen

Human IgM

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

IM373

Conjugation

CF740

Disease

Tumor

Source

Animal

Applications

IHC, FFPE (verified) | WB (verified)

Validated Applications

IHC,FFPE,WB

Field of Research

Cancer, Immunology, Inflammation

Positive Control

293T, Raji or hPBL cells. Tonsil or Spleen

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

50-75 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/11642558/datasheet/bnc740373-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/11642558/msds/bnc740373-500.pdf

CAS Number

9007-83-4

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