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Anti-AFP (Alpha Fetoprotein)(C3), CF740 conjugate

Alpha-fetoprotein (AFP) is an oncofetal glycoprotein with a single chain of 70 kDa. This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Alpha-fetoglobulin; FETA; HPAFP

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

AFP

Gene ID

174

NCBI Gene ID

518808

UniProt

P02771

Cellular Locus

Cytoplasmic|Golgi apparatus

Host

Mouse

Species Reactivity

Dog,Human,Monkey,Pig

Immunogen

Alpha fetoprotein (AFP) purified from serum of a hepatoma patient

Target Antigen

AFP | Alpha-fetoprotein

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

C3

Conjugation

CF740

Disease

Tumor

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Field of Research

Cancer, Developmental biology

Positive Control

Hep-G2 cells. Fetal liver or hepatocellular carcinoma

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

70 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 1-2 ug/mL|Does not react with cow, dog, mouse, or rat|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/11642537/datasheet/bnc740354-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/11642537/msds/bnc740354-500.pdf

CAS Number

9007-83-4

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