Steady-Luc™ Firefly HTS Assay Kit (Lyophilized), 1000 Assays

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples. </p>Biotium's Steady-Luc trade; HTS assay system is a proprietary mixture of substances that modify the enzymatic reaction to produce a long lasting signal (steady glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a homogeneous high sensitivity firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates. </p>Glow-type luciferase assays like Steady-Luc trade; have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity. </p>The Steady-Luc trade; Firefly HTS Assay Kit (Lyophilized) is comparable to our original Steady-Luc trade; HTS Assay, but with assay buffer provided in lyophilized format for economical shipping and convenient storage. Simply dissolve the lyophilized assay buffer in the supplied reconstitution buffer before use. </p><p class=" quot;style5"><strong>Kit Components: </strong></p> </p><ul> </p> <li>D-Luciferin</li> </p> <li>Steady-Luc Firefly Assay Buffer (Lyophilized)</li> </p> <li>Reconstitution Buffer</li> </p></ul> </p><strong>Features:</strong> </p><ul> </p> <li><b>Simple:</b> single step homogeneous assay.</li> </p> <li><strong>Glow kinetics:</strong> luminescence half life of about 3 hours.</li> </p> <li><b>Convenient:</b> lyophilized assay buffer for convenient shipping and storage.</li> </p> <li><b>Economical:</b> save up to 45% compared to other brands.</li> </p></ul>