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BMI1 Recombinant Protein

Product Specifications

Gene Name

BMI1 proto-oncogene, polycomb ring finger|COMMD3-BMI1 readthrough

Gene Aliases

B lymphoma Mo-MLV insertion region 1 homolog; BMI1; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; COMMD3-BMI1 read-through protein; flvi-2/bmi-1; FLVI2/BMI1; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group RING finger protein 4; ring finger protein 51; RNF51.

Gene ID

100532731|648

Accession Number

NP_001190991.1

Reactivity

Homo sapiens|Human

Target

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373) . The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358) . In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249) .

Conjugation

Unconjugated

Type

Protein

Source

E.coli

Applications

Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

200 ug/mL (prior to lyoph)

Format

Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Reconstitution

2°C to 8°C|-80°C

Molecular Weight

24kDa

Protein Length

Recombinant

NCBI Gene Symbol

BMI1|COMMD3-BMI1

Host or Source

E.coli

Protein Name

Polycomb complex protein BMI-1

Gene Name URL

BMI1|COMMD3-BMI1

CAS Number

9000-83-3

Nucleotide Accession Number

NM_001204062.1

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