SETX ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
Gene Name
Senataxin
Gene Aliases
A; A130090N03; A930037J23Rik; Als4; amyotrophic lateral sclerosis 4 homolog; amyotrophic lateral sclerosis 4 protein homolog; AOA2; AW060766; mKIAA0625; probable helicase senataxin; SCAR1; Sen1; SEN1 homolog.
Gene ID
269254
Accession Number
NP_932150.2
Reactivity
Mouse, Mus musculus
Target
Probable helicase senataxin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology SETX ELISA Kit (OKEH08818) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-SETX antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SETX and incubated. The SETX found in the sample or standards competes with the biotinylated SETX for limited binding sites on the immobilized anti-SETX antibody. Excess unbound biotinylated SETX and sample or standard SETX is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SETX captured in the well and inversely proportional to the amount of SETX which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)
Sensitivity
0.23ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Probable helicase senataxin
NCBI Gene Symbol
Setx
Protein Name
A2AKX3
Gene Name URL
Setx
CAS Number
7732-18-5
Nucleotide Accession Number
NM_198033.2
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